Anti-Chymase Antibody, clone B7, biotin conjugated

32160702

NA.41

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Reacts with human mast cell chymase.

Chymase purified from human skin.

Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.
Immunohistochemistry at 1:2,000:1:4,000.

For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology

Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

biotin conjugate

culture supernatant

primary antibodies

B7, monoclonal

human

Chemicon^®

dot blot: suitable
immunohistochemistry: suitable

IgG1κ

not suitable for Western blot

NM_001836.2

P23946

wet ice

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2