Anti-Collagen Type IV Antibody

32160702

NA.41

Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Human and bovine placental collagen type IV

Research Category
Cell Structure
Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.
Research Sub Category
ECM Proteins
Dot and slot blotting: 1:100-1:500

ELISA: 1:1000-1:4000

Indirect immunohistochemistry (frozen sections only): 1:10-1:40

Immunocytochemistry 1:10-1:20

Optimal working dilutions must be determined by the end user.

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose^® 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.
ImmunoAffinity Purified

Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a registered trademark of Cytiva

100

goat

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

human, mouse, bovine

Chemicon^®

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)

NM_001845.4

P02462

wet ice

human ... COL4A1(1282)

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

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