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Anti-Fascin Antibody, clone 55K2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| Specificity【特异性】 | Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones. |
| Immunogen【免疫原】 | Human fascin |
| Application【应用】 | Research Category Cell Structure Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS. Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000. Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000. Optimal working dilutions must be determined by the end user. Research Sub Category Cytoskeleton This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin. |
| Physical form【外形】 | Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide. Format: Purified |
| Analysis Note【分析说明】 | Control POSITIVE CONTROL: Hela cells, neuronal tissues, antigen presenting dendritic cells. |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 55K2, monoclonal |
| species reactivity | gerbil, human, sea urchin, echinoderm |
| species reactivity (predicted by homology) | mammals |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
| isotype【同位素/亚型】 | IgG1 |
| suitability【适用性】 | not suitable for immunoprecipitation |
| NCBI accession no.【NCBI登记号】 | NM_003088.2 |
| UniProt accession no.【UniProt登记号】 | Q16658 |
| shipped in【运输】 | wet ice |
产品说明
| Storage and Stability【储存及稳定性】 | Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
