Anti-Fibroblast activation protein alpha (FAP) Antibody

32160702

NA.41

Fibroblast activation protein alpha (FAP), also known as Seprase, is a 97 kDa, type II cell surface glycoprotein belonging to the serine protease family. Although FAP is considered fibroblast specific, it is not found in normal healthy fibroblasts. FAP is most commonly found in stromal, tumor fibroblasts, and is likely involved in ECM pericellular proteolysis, endothelial cell migration and invasion into the ECM. The presence of FAP in human epithelial carcinoma is increased, particularly in breast, colorectal, lung, and ovarian carcinomas. FAP is also transiently observed in developing cells, or in tissues that are undergoing wound healing or suffering from chronic inflammatory conditions. Due to its specific distribution both to tumor fibroblasts and to inflammed tissue, FAP makes a promising target for research in tumor-stromal studies and in therapeutic studies.

The antibody recognizes the extracellular domain of Fibroblast activation protein alpha

Epitope: Extracellular domain
Histidine-tagged recombinant protein corresponding to human FAP.

Research Category
Cell Structure

Apoptosis & Cancer
Research Sub Category
ECM Proteins

Tumor Markers
Immunohistochemistry: A 1:750 dilution of a previous lot of FAP antibody was used to immunohistochemically stain frozen tissue sections of HEK-FAP or HT-29 xenograft tumors from C.B17 mice (Cheng, 2002).

Inhibition: Inhibition of dipeptidyl peptidase activity with a previous lot of FAP antibody on HT-29 xenograft tumors was demonstrated by an independent laboratory (Cheng, 2002).
Detect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.

Evaluated by Western Blot in HEK-FAP xenograft tumor lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected FAP in 10 µg of HEK-FAP xenograft tumor lysate.

Ammonium Sulfate
Format: Purified
Purified rabbit polyclonal in buffer containing PBS (pH 7.2) with 0.05% sodium azide.

Control
HEK-FAP xenograft tumor lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

saturated ammonium sulfate (SAS) precipitated

primary antibodies

polyclonal

human

immunohistochemistry: suitable
western blot: suitable

IgG

NP_004451

Q12884

wet ice

~ 90 kDa observed MW. Two isoforms of the Fibroblast activation protein exist at calculated MWs of 88kDa and 27 kDa It is possible that both bands may be obserbved in certain cell lines.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1