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Anti-FIP200 Antibody, clone 14E11.2
产品别名
Anti-FIP200 Antibody, clone 14E11.2
RB1-inducible coiled-coil protein, 1FAK family kinase-interacting protein of 200 kDa, FIP200
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Focal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK. |
| Specificity【特异性】 | This antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions. |
| Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to the interaction domain of human FIP200. Epitope: Interaction domain |
| Application【应用】 | Western Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells. Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells. Research Sub Category Apoptosis - Additional Research Category Apoptosis & Cancer Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC. |
| Quality【质量】 | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate. |
| Physical form【外形】 | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
| Analysis Note【分析说明】 | Control HeLa cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 14E11.2, monoclonal |
| species reactivity | human, mouse |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG2bκ |
| NCBI accession no.【NCBI登记号】 | NP_001077086 |
| UniProt accession no.【UniProt登记号】 | Q86YR4 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... RB1CC1(9821) |
产品说明
| Target description【目标描述】 | ~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
