Anti-Glutamine Synthetase Antibody, clone GS-6

32160702

NA.41

Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.

Reacts strongly with a single band on Western blots of sheep brain and rat brain. Glutamine synthetase, an enzyme that catalyzes the amination of glutamic acid to form glutamine, is found in mammals as an octamer of eight identical 45 kDa subunits. Glutamine synthetase activity has been shown to be a useful marker of astrocytes and an important differentiation feature in retina. Glutamine synthetase is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.

Glutamine synthetase purified from sheep brain.

Research Sub Category
Neurofilament & Neuron Metabolism
Western blot: 1:1,000-1:5,000

Immunohistochemistry on rat brain

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Detect Glutamine Synthetase using this Anti-Glutamine Synthetase Antibody, clone GS-6 validated for use in ELISA, IH, IH(P) & WB with more than 45 product citations.

Format: Purified
Protein A purified
Purified immunoglobulin. Liquid in buffer containing 50% glycerol, BSA and 0.09% sodium azide.

Control
Rat brain tissue, rat brain cytosolic fraction extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

GS-6, monoclonal

sheep, human, rat, mouse

Chemicon^®

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG2a

NM_001033044.1

P15104

dry ice

human ... GLUL(2752)

45 kDa

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2