Anti-GPI Antibody, clone 1B7D7

32160702

NA.41

Glucose-6-phosphate isomerase (GPI) alternatively known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI) or Sperm antigen 36 (SA-36) and encoded by the gene name GPI is major enzyme in glycolysis and gluconeogenesis. Glucose-6-phosphate isomerase rearranges glucose-6-phosphate (from the phosphorylation of glucose via hexokinases) into fructose-6-phosphate (F6P) as the second step in glycolysis. Interestingly however, Glucose-6-phosphate isomerase plays an entirely different sort of role outside of the cell than when it is inside being part of glycolysis. When secreted, Glucose-6-phosphate isomerase acts as an autocrine motility factor for various cancers and is critical for metastasis, while when secreted in nervous tissue it acts as a neurotrophic factor for sensory and spinal neurons and in lymphocytes it acts as a lymphokine upon T cell ligand binding and stimulates B cells to secrete immunoglobulin. Mutations in Glucose-6-phosphate isomerase are the second most frequent cause of inherited glycolytic-enzymopathy in humans. This autosomal recessive disorder is characterized by anon-spherocytic anemia of variable severity which can present with neuromuscular dysfunctions defined by muscle weakness and mental retardation. EMD-Millipore’s Anti-Glucose-6-phosphate isomerase monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HepG2, SMMC-7721 and rat liver tissue and in paraffin embedded immunohistochemistry on human kidney and fluorescent immunocytochemistry on L-O2 cells in culture.

Purified recombinant fragment of human GPI expressed in E. Coli.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in human kidney tissue.

Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in L-02 cells.

Optimal working dilutions must be determined by end user.
Anti-GPI Antibody, clone 1B7D7 is a highly specific mouse monoclonal antibody, that targets Glucose-6-phosphate isomerase & has been tested in western blotting, IHC & Immunofluorescence.

Evaluated by Western Blotting in HepG2, SMMC-7721, and rat liver lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPI in HepG2, SMMC-7721, and rat liver lysates.

Unpurified

100

mouse

ascites fluid

primary antibodies

1B7D7, monoclonal

human, rat

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1

P06744

wet ice

human ... NLK(51701)

~56 kDa observed. Uncharacterized bands may appear in some lysate(s).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

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