Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

32160702

NA.41

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.

Recombinant mouse HP1alpha.

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
ELISA: 1:500-1:5,000

Western blot: 1:500-1:5,000

Immunoprecipitation: 1:500-1:5,000

Immunohistochemistry/Immunocytochemistry: Not Recommended

Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.

Optimal working dilutions must be determined by the end user.

Liquid ascites fluid containing no preservative.
Unpurified

Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

ascites fluid

primary antibodies

2HP-2G9, monoclonal

mouse, human

Chemicon^®

ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1

NM_012117.1

P45973

wet ice

human ... CBX5(23468)

25 kDa

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1