Anti-Interferon-α2 Antibody, clone ST2541

32160702

NA.41

Interferon alpha-2 (UniProt P01563; also known as IFN-alpha-2, Interferon alpha-A, LeIF A) is encoded by the IFNA2 (also known as IFNA2A, IFNA2B, IFNA2C) gene (Gene ID 3440) in human. Interferons (IFNs) are cytokines whose prouction and release by host cells are induced in the presence of pathogens (e.g. viruses, bacteria, parasites) and tumor cells. Originally named for their ability to interfere with viral replication, IFNs mediate the activate immune cells, such as natural killer cells and macrophages, and increase host defense by up-regulating antigen presentation by boosting the expression of major histocompatibility complex (MHC) antigens. More than twenty distinct IFN genes and proteins have been identified and classified into three main types. Type I IFNs (IFN-α, IFN-β, IFN-ε, IFN-κ, and IFN-ω) exert their function via binding IFN-α/β receptor (encoded by the IFNAR1 and IFNAR2 gene, respectively). IFN-γ is the only known type II IFN, it is released by type 1 T helper cells and binds IFN-γ receptor (encoded by the IFNGR1 and IFNGR2 gene, respectively). Type III IFNs signal through a receptor complex consisting of IL10R2 (CRF2-4) and IFNLR1 (CRF2-12).

Clone ST2541 neutralized human IFN-αA (IFN-α2) and IFN-αN (produced by Nalmalwa human Burkitt′s lymphoma cells), but not IFN-α1, antiviral activity (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).

Human alpha-interferon.

Research Sub Category
Cytokines & Cytokine Receptors
Neutralizing Analysis: A representative lot enhanced viral replication by neutralizing host-secreted IFN in human prostate cancer PC3 cultures infected with P/V-CPI(−) and P/V-CPI(−)-G3A at a low MOI (Gainey, M.D., et al. (2000). J. Virol. 82(19):9369-9380).
Neutralizing Analysis: A representative lot neutralized human IFN-αA (IFN-α2) and IFN-αN (produced by Nalmalwa human Burkitt′s lymphoma cells), but not IFN-α1, antiviral activity against Semliki Forest Virus in human WISH amniotic epithelial cell cultures (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
Affinity Binding Assay: A representative lot captured human IFN-α2, but not human IFN-α1 (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
ELISA Neutralizing Analysis: A representative lot was used as the detection antibody for the detection of human IFN-αN (produced by Nalmalwa human Burkitt′s lymphoma cells) by sandwich ELISA (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
Western Blotting Analysis: A representative lot detected IFN-αA (IFN-α2) and IFN-αN (produced by Nalmalwa human Burkitt′s lymphoma cells) under reducing condition by Western blotting (Shearer, M., et al. (1984). J. Immunol. 133(6):3096-3101).
Detect Interferon-α2 using this Anti-Interferon-α2 Antibody, clone ST254 validated for use in NEUT, WB, ELISA, Affinity binding.
Research Category
Inflammation & Immunology

Liquid, no preservatives

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

ascites fluid

primary antibodies

ST2541, monoclonal

human

Chemicon^®

ELISA: suitable
affinity binding assay: suitable
neutralization: suitable
western blot: suitable

IgG1

NM_000605.2

P01563

dry ice

Store at -20°C for up to one year. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1