您的位置：首页 > 产品中心 > Anti-LCoR (Splice Variant)
Anti-LCoR (Splice Variant)
产品别名
Anti-LCoR (Splice Variant)
Ligand-dependent corepressor, Mblk1-related protein 2, C10ORF12, LCoR (splice variant), PALI1
基本信息
| eCl@ss | 32160702 |
| General description【一般描述】 | Ligand-dependent corepressor (UniProt: Q96JN0; also known as LCoR, Mblk1-related protein 2) is encoded by the LCOR (also known as C10orf12, KIAA1795, MLR2) gene (Gene ID: 84458) in human. LCoR is a ubiquitously distributed protein in fetal and adult tissues and serves as a transcriptional co-repressor that is recruited to agonist-bound nuclear receptors through a single LxxLL motif at the N-terminal region. It may also act as a transcription activator that binds DNA elements with the sequence 5′-CCCTATCGATCGATCTCTACCT-3′. Three isoforms of LCoR have been described that are produced by alternative splicing. This polyclonal antibody is raised against a large isoform (isoform 3; a splice variant), which associates with the PRC2 complex through its C-terminal region. Its nuclear localization signal is reported to be in amino acids 339-345 and it reacts with nuclear receptors via amino acids 53-57. (Ref.: Conway, E., et al. (2018). Mol. Cell 70(3); 408-421). |
| Specificity【特异性】 | This rabbit polyclonal antibody detects a splice variant of human and murine Ligand-dependent corepressor (LCoR). It targets an epitope with in the N-terminal half. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human Ligand-dependent corepressor (LCoR), isoform 3. Epitope: unknown |
| Application【应用】 | Anti-LCoR, Cat. No. ABE1367, is a highly specific rabbit polyclonal antibody that targets a splice variant form of Ligand-dependent corepressor (LCoR) and has been tested in Immunocytochemistry and Western Blotting. Research Category Epigenetics & Nuclear Function Western Blotting Analysis: A 1:500 dilution from a representative lot detected LCoR in whole cell lysates from Pali1 KO and matched wild type mouse embryonic stem (Courtesy of Dr. Eric Conway, Dr. Emilia Jerman & Prof. Adrian P. Bracken at the Smurfit Institute of Genetics, Trinity College Dublin, Ireland). Western Blotting Analysis: A representative lot detected LCoR in Western Blotting applications (Conway, E., et. al. (2018). Mol. Cell. 70(3):408-421). |
| Quality【质量】 | Evaluated by Immunocytochemistry in NTERA2 cells. Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LCoR in NTERA2 cells. |
| Physical form【外形】 | Format: Purified Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity Purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | mouse, human |
| packaging【包装】 | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| NCBI accession no.【NCBI登记号】 | NP_001333445 |
| UniProt accession no.【UniProt登记号】 | Q8N655 Q96JN0 Q96JN0-3 |
| Gene Information | human ... LCOR(84458) |
产品说明
| Target description【目标描述】 | ~250 kDa observed; 170.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
