Anti-LEF-1 Antibody, all isoforms, clone 1C3.1D10

32160702

Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 256-308 of LEF-1. This region is between the transactivation domain and the HMG binding domain of LEF-1 and is conserved in all LEF-1 isoforms.

Recognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein. Does not cross-react with TCF-1 or TCF-4.

Recombinant full length LEF-1 protein.
Epitope: all isoforms

Research Sub Category
Neural Stem Cells

Hematopoietic Stem Cells
Anti-LEF-1 Antibody, all isoforms, clone 1C3.1D10 is an antibody against LEF-1 for use in WB.
Western blot

Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research

Replaces: 04-1130

Format: Purified
Purified immunoglobulin. Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Control
Jurkat cell lysate and human colon adenocarcinoma tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

1C3.1D10, monoclonal

human

Chemicon^®

western blot: suitable

sample type hematopoietic stem cell(s)

IgG1

NM_016269.2

Q9UJU2

dry ice

human ... LEF1(51176)

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2