Anti-Lunapark Antibody

32160702

NA.41

Protein lunapark (UniProt Q9C0E8; also known as Ulnaless) is encoded by the LNP (also known as KIAA1715) gene (Gene ID 80856) in human. The endoplasmic reticulum (ER) consists of a polygonal network of sheets and tubules interconnected by three-way junctions that undergoes continual remodeling through two competing processes. The branching and fusion of tubules lead to the formation of new three-way junctions and polygons, while junction sliding and ring closure lead to polygon loss. Both yeast Lnp1 and mammalian lunapark (mLnp1) are reported to play an important role in the maintenance of ER morphology through junctions stabilization. Study conducted in COS-7 cells demonstrated that junctions with lunapark are less mobile than junctions lacking lunapark. New junctions acquired lunapark tend to remain stable within the ER network, whereas the nascent junctions that fail to acquire lunapark preferentially undergo ring closure.

Exprected to react with all four spliced isoforms of human lunapark reported by UniProt (Q9C0E8).

GST-tagged full-length recombinant human Lunapark.

Detect Lunapark using this Anti-Lunapark Antibody validated for use in Western Blotting.
Western Blotting Analysis: The purified antibody was used to detect Lunapark expression in COS-7 cells and confirm siRNA-medidated Lunapark downregulation (Chen, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(2):418-423).
Research Category
Signaling
Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Lunapark in 10 µg of HeLa cell lysate.

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Concentration: Please refer to lot specific datasheet.

rabbit

100

serum

primary antibodies

polyclonal

monkey, human

western blot: suitable

NP_085153

Q9C0E8

dry ice

human ... KIAA1715(80856)

~47 kDa observed. 47.74 kDa (isoform 1), 47.09 kDa (isoform 2), 33.01 kDa (isoform 3), and 50.85 kDa (isoform 4) calculated.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1