Anti-Methyl-Glutamine

32160702

NA.41

Glutamine methylation occurs on translation termination factors and ribosomal proteins. Protein methylation has been reported to affect various cellular processes including protein stability, protein-protein interaction, and protein localization. Methylation of histone is suggested as a platform to recruit and regulate other chromatin-related factors. Glutamine methylation is brought about by the action of protein glutamine methyltransferase HEMK2 that specifically requires a GQX3R motif for methylation activity. This antibody was tested for glutamine methylation in chromodomain helicase DNA-binding protein 5 (CHD5) that contains multiple PHD and chromo domains and is reported to function as a tumor suppressor.

This rabbit polyclonal antibody detects methylglutamine in proteins.

KLH-conjugated Gly-Gln (Me)-Gly tripeptide.

Anti-Methyl-Glutamine, Cat. No. ABS2185, is a rabbit polyclonal antibody that detects glutamine methylated proteins. It has been tested for use in Western Blotting.
Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Methyl-Glutamine in CHD5 unmethylated & CHD5 methylated recombinant lysate (Coutesy of, Dr. Kusevic, Dr. Kuditipudi and Dr. Jeltsch at University Stuttgart, Germany).

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Methyl-Glutamine in in-vitro methylated CHD5, NUT, and eRF1 proteins (Kusevic, D., et. al. (2016). J Biol Chem. 291(12):6124-33).

Evaluated by Western Blotting in methylated recombinant chromodomain helicase DNA-binding 5 (CHD5) protein, but not in unmethylated recombinant CHD5.

Western Blotting Analysis: A 1:3,000 dilution of this antibody detected Methyl-Glutamine in methylated recombinant chromodomain helicase DNA-binding 5 (CHD5) protein, but not in unmethylated recombinant CHD5.

Format: Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.

Antiserum was depleted by running over unmethylated protein column.

Concentration: Please refer to lot specific datasheet.

rabbit

100

unpurified

primary antibodies

polyclonal

all

antibody small pack of 25 μL

western blot: suitable

IgG

ambient

~51 kDa observed (for methylated CHD5). Uncharacterized bands may be observed in some lysate(s).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1