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Anti-MyoD Antibody, clone 5F11
产品别名
Anti-MyoD Antibody, clone 5F11
Myoblast determination protein 1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Myoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5. |
| Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to mouse MyoD. |
| Application【应用】 | Immunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells. Western Blot Analysis: A representative lot detected MyoD in C2C12 cells. Research Category Signaling Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC. Research Sub Category Developmental Signaling |
| Quality【质量】 | Evaluated by Western Blot in C2C12 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate. |
| Linkage【联系】 | Replaces: MABE514 |
| Physical form【外形】 | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
| Analysis Note【分析说明】 | Control C2C12 cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | rat |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 5F11, monoclonal |
| species reactivity | mouse |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG2aκ |
| NCBI accession no.【NCBI登记号】 | NP_034996 |
| UniProt accession no.【UniProt登记号】 | P10085 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
