Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb

rabbit

100

primary antibodies

polyclonal

liquid

≤0.1% sodium azide as preservative

human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG

wet ice

−20℃

100 μL in Plastic ampoule

Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.
This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).
Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.

a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
Human

ELISA (see comments)

Gel Shift (see comments)

Immunoblotting (1:500, chemiluminescence)

Immunoprecipitation (see comments)

Toxicity: Standard Handling (A)

In 5 mM sodium phosphate buffer, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
Daudi cells

Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.
This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1