Anti-NLRC4 antibody produced in rabbit

HPA006592    Human Protein Atlas characterization data

NLRC4 (NLR family, CARD domain containing 4) is a cytoplasmic immune receptor protein associated with inflammatory responses. It is a member of the nucleotide-binding site and leucine-rich repeat (NBS-LRR) protein. It consists of ATP/GTPase-specific P-loop and Mg²⁺-binding site motifs. The NBs domain contains a nucleotide-binding pocket for ATP/dATP.

NLR family, CARD domain containing 4 recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

NLRC4 (NLR family, CARD domain containing 4) is involved in the inflammatory responses. Activated NLR (NOD-like receptors) transports the caspase-1 within the inflammasome for its proteolytic activation. It converts the inactivated pro-IL-1 into active caspase by autoproteolysis. This multiprotein caspase-1 generates several innate immune sensors such as interleukin-1 family cytokines (IL1FCs), followed by cytokine productions and inflammatory cell death (pyroptosis). Mutation in the NLRC4 causes an auto-inflammatory syndrome of enterocolitis and autoinflammation.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST70783.

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

immunohistochemistry: 1:500- 1:1000

TDSLGNLKNLTKLIMDNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWG

Q9NPP4

wet ice

−20℃

human ... NLRC4(58484)

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12 - Non Combustible Liquids

WGK 1