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Anti-Notch 1 Antibody, extracellular, clone 8G10
产品编号: | 4111347 |
规格: | culture supernatant, clone 8G10, Chemicon® |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-Notch 1 Antibody, extracellular, clone 8G10
Motch A, mT14, p300
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Notch 1 is a type 1 membrane protein that serves as a receptor for the membrane bound ligands jagged-1 and -2 and delta-1 to regulate cell fate determination through the implementation of differentiation, proliferation and apoptotic programs. Upon ligand binding, the Notch intracellular domain (NICD) is released by proteolytic processing and subsequently translocates to the nucleus where it forms a transcriptionally active complex with RBP-J kappa to activate genes of the enhancer of split locus. Notch-1 may also be involved in cell specification and/or differentiation and body segment determination in the postimplantation embryo. In the adult animal, Notch-1 is highly expressed in the brain, lung, and thymus with lower expression levels in the spleen, bone-marrow, spinal cord, eyes, mammary gland, liver, intestine, skeletal muscle, kidney and heart. |
Specificity【特异性】 | Reacts with natural and recombinant Notch1. Recognizes a band migrating at approximately 300 kDa on Western blots of rat and mouse protein extracts. A cross reactive band migrating at approximately 230 kDa is observed in some samples and is believed to correspond to the processed extracellular fragment of Notch 1. |
Immunogen【免疫原】 | A GST fusion protein comprising amino acids 1299-1492 of mouse Notch 1 Epitope: extracellular |
Application【应用】 | Research Sub Category Transcription Factors Western blot Immunohistochemistry on paraformaldehyde fixed frozen tissue sections. Flow Cytometry Optimal working dilutions must be determined by end user. Research Category Epigenetics & Nuclear Function Use Anti-Notch 1 Antibody, extracellular, clone 8G10 (Syrian Hamster Monoclonal Antibody) validated in FC, WB, IHC to detect Notch 1 also known as Motch A, mT14, p300. |
Linkage【联系】 | Replaces: 04-1046 |
Physical form【外形】 | Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2 micron membrane prior to vialing. Product contains 20% FBS and Ciprofloxacin at final concentration of 10 μg/mL. |
Analysis Note【分析说明】 | Control POSITIVE CONTROL: brain, lung, thymus. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | hamster (Syrian) |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | culture supernatant |
antibody product type | primary antibodies |
clone【克隆】 | 8G10, monoclonal |
species reactivity | rat, mouse |
manufacturer/tradename | Chemicon® |
technique(s) | flow cytometry: suitable immunohistochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1 |
NCBI accession no.【NCBI登记号】 | NM_017617.3 |
UniProt accession no.【UniProt登记号】 | P46531 |
shipped in【运输】 | wet ice |
Gene Information | human ... NOTCH1(4851) |
产品说明
Storage and Stability【储存及稳定性】 | Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |