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Anti-phospho-DBC1 (Thr454) Antibody
| 产品编号： | 4112311 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho-DBC1 (Thr454) Antibody
DBIRD complex subunit KIAA1967, Deleted in breast cancer gene 1 protein, DBC-1, DBC.1, p30 DBC
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Deleted in breast cancer gene 1 protein (DBC1) is a large, nuclear protein with multiple functions in cell survival that binds directly to the estrogen receptor α (ERα) hormone-binding domain in a strict ligand-independent manner. DBC1 promotes p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity. It is ubiquitously expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. |
| Specificity【特异性】 | This antibody recognizes DBC1 phosphorylated at Thr454. |
| Immunogen【免疫原】 | Epitope: Phosphorylated Thr454 KLH-conjugated linear peptide corresponding to human DBC-1 phosphorylated at Thr454. |
| Application【应用】 | Peptide Inhibition Assay: A 1:500 dilution from a representative lot blocked DBC-1 phosphorylated at Thr454 in untreated and etoposide treated Jurkat cell lysates. Research Category Signaling Anti-phospho-DBC1 (Thr454) Antibody is an antibody against phospho-DBC1 (Thr454) for use in Western Blotting, Peptide Inhibition Assay. Research Sub Category Developmental Signaling |
| Quality【质量】 | Evaluated by Western Blot in untreated and etoposide treated Jurkat cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected DBC-1 phosphorylated at Thr454 in 10 µg of etoposide treated Jurkat cell lysates. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control Untreated and etoposide treated Jurkat cell lysates |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| technique(s) | inhibition assay: suitable (peptide) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_066997 |
| UniProt accession no.【UniProt登记号】 | Q8N163 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~130 kDa observed. Uniprot states the calculated molecular weight is 103 kDa but has been shown at ~130kDa (Satoh, M., et al. (1995). Brain Res Dev Brain Res. 87(2):111-119). |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
