Anti-phospho-Glucocorticoid receptor Antibody, Ser226

32160702

NA.41

Glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1, and encoded by the gene name NR3C1/ GRL, is receptor for glucocorticoids (GC). GR has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. GR affects inflammatory responses, cellular proliferation and differentiation in target tissues. Recent studies have shown that GR may act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. In addition, GR is involved in chromatin remodeling, and may play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. Mutation in GR has been associated with Glucocorticoid resistance (GCRES): a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. GR is widely expressed. In the heart, GR is detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart.

KLH-conjugated linear peptide corresponding to the domain of: Modulating region of Human Glucocorticoid receptor.
Epitope: A region within the modulating domain

Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in transfected U2OS cell lysate (Chen, W., et al. Mol Endocrinol, August 2008, 22(8):1754–1766)
Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in rat hepatoma cell lysate (Blind,R., et al. Journal of Steroid Biochemistry & Molecular Biology 109 (2008) 150–157)

Research Sub Category
Lipid Metabolism & Weight Regulation
Research Category
Signaling
This Anti-phospho-Glucocorticoid receptor antibody is validated for use in Western Blotting for the detection of phospho-Glucocorticoid receptor.

Evaluated by Western Blotting on transfected HEK293 cell lysate

Western Blotting Analysis: 1 µg/mL dilution of this antibody detected phosphorylated glucocorticoid receptor in 6 µg of transfected HEK293 cell lysate

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Antigen Affinity Purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

rat (based on 100% sequence homology), mouse (based on 100% sequence homology)

western blot: suitable

NP_000167

P04150

wet ice

human ... NR3C1(2908)

~85 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2