Anti-phospho-ULK1 (Ser555) Antibody

32160702

NA.41

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

This antibody recognizes ULK1 phosphorylated at Ser555.

KLH-conjugated linear peptide corresponding to human ULK1 phosphorylated at Ser555.
Epitope: Phosphorylated Ser555

Peptide Inhibition Analysis: A representative lot of this antibody blocked ULK1 in PIA involving peptide 3123-C vs. peptide 3123 on serum starved MCF7 cell lysates.
This Anti-phospho-ULK1 (Ser555) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay for the detection of phospho-ULK1 (Ser555).
Research Category
Apoptosis & Cancer
Research Sub Category
Kinases & Phosphatases

Evaluated by Western Blot in non-serum starved and serum starved MCF7 cell lysates.

Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-ULK1 (Ser555) in 10 µg of non-serum starved and serum starved MCF7 cell lysates.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
Non-serum starved and serum starved MCF7 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

mouse (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)

inhibition assay: suitable (peptide)
western blot: suitable

NP_003556

O75385

wet ice

~120 kDa observed. Uniprot describes a molecular weight at 112 kDa This protein has been observed at greater than ~125 kDa due to multiple phosphorylation-dephosphorylation processes. (Ganley, I. G., et al. (2009). J Biol Chem. 284(18):12297-12305.)

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1