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Anti-Phosphothreonine Antibody, clone RM102
| 产品编号： | 4112761 |
| 规格： | clone RM102, from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Phosphothreonine Antibody, clone RM102
p-Thr, pthr, phospho-threonine
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. |
| Immunogen【免疫原】 | Mix of recombinant protein and peptide corresponding to All Phosphothreonine. |
| Application【应用】 | Immunocytochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on Calyculin A/Okadaic Acid treated A431 cells Immunohistochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on human colon cancer tissue Immunoprecipitation Analysis: A representative lot of this antibody at 1:500 dilution immunoprecipitated phosphothreonine modified proteins from Calyculin A/Okadaic Acid treated A431 cell lysate Anti-Phosphothreonine, clone RM102 is an antibody against Phosphothreonine for use in WB, ICC, IH, IP. |
| Quality【质量】 | Evaluated by Western Blotting on Calyculin A/Okadaic Acid treated HeLa cell lysate Western Blotting Analysis: A 1:2000 dilution of this antibody detected phosphothreonine modification on Calyculin A/Okadaic Acid treated HeLa cell lysate |
| Other Notes【其他说明】 | Protein A purified |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | RM102, monoclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | all |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | N/A kDa observed |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
