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Anti-Proliferating Cell Nuclear Antigen antibody, Mouse monoclonal

产品编号:4113170
规格:clone PC 10, purified from hybridoma cell culture
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4113170100 μL6730
产品别名

Anti-Proliferating Cell Nuclear Antigen antibody, Mouse monoclonal

Anti-ATLD2

Anti-Cyclin

Anti-PCNA

基本信息
NACRES
NA.41
General description【一般描述】
PCNA (proliferating cell nuclear antigen) gene is mapped in human chromosome 20p12.3. PCNA is a homotrimeric protein, which is expressed in the nucleus. The PCNA ring has a large central cavity that allows double-stranded DNA to slide freely along it.
Anti-Proliferating Cell Nuclear Antigen antibody (mouse IgG2a isotype) is derived from the PC 10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PCNA is an auxiliary protein of DNA polymerase δ and belongs to the conserved sliding clamp family of proteins.
Specificity【特异性】
Monoclonal Anti-Proliferating Cell Nuclear Antigen (PCNA) specifically recognizes Proliferating Cell Nuclear Antigen from human, monkey, rat, mouse, insect and yeast origin.
Immunogen【免疫原】
PCNA-Protein A fusion protein.
Application【应用】
Anti-Proliferating Cell Nuclear Antigen antibody, Mouse monoclonal may be used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • flow cytometry
  • immunohistochemistry
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions【生化/生理作用】
PCNA protein is essential for chromosomal DNA replication during S-phase and is required for several DNA processes like DNA repair, sister chromatid cohesion and cell cycle control. This protein is also involved in the epigenetic modification and chromatin assembly and remodeling. PCNA acts as a prognostic and diagnostic marker in several diseases such as breast cancer, chronic lymphoid leukemia, non-Hodgkin′s lymphoma, malignant and nonmalignant skin diseases and systemic lupus erythematosus.
PCNA (proliferating cell nuclear antigen) maintains the polymerase and DNA association by forming a sliding clamp and ensures efficient DNA replication. PCNA is responsible for recruiting important regulatory factors for DNA damage tolerance mechanism. In response to UV irradiation-induced DNA damage, monoubiquitination of PCNA mediates the polymerase switching model by recruiting DNA polymerase η to PCNA. The protein is involved in base excision repair and maintains genome stability. PCNA is associated with proteolysis coupled to DNA synthesis and repair for proper cell cycle progression.
Physical form【外形】
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
antibody form【抗体形式】
purified from hybridoma cell culture
antibody product type
primary antibodies
clone【克隆】
PC 10, monoclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen ~36 kDa
species reactivity
yeast, human, monkey, insect, rat, mouse
concentration【浓度】
~1.0 mg/mL
technique(s)
ELISA: suitable
flow cytometry: suitable
immunoblotting: 0.5-1 μg/mL using human foreskin fibroblast HS-68 cell line extract
immunofluorescence: 1.25-2.5 μg/mL using HeLa cells
immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Tonsil sections.
immunoprecipitation (IP): suitable
isotype【同位素/亚型】
IgG2a
UniProt accession no.【UniProt登记号】
P12004
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
产品说明
Storage and Stability【储存及稳定性】
For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
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