Anti-PTEN mono-ubiquitylated K13

32160702

NA.41

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (UniProt: P60484; also known as Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN) is encoded by the PTEN (also known as MMAC1, TEP1) gene (Gene ID: 5728) in human. PTEN belongs to the family of lipid phosphatases. It contains an N-terminal phosphatase catalytic domain, a C2-domain that binds to phospholipid membranes, and a C-terminal regulatory tail. It is a dual-specific phosphatase, which dephosphorylates both lipid and protein substrates. PTEN blocks the phosphatidylinositol 3-kinase/Akt signaling pathway by removing the phosphate in the 3-position on phosphoinositide-3, 4, 5-trisphosphate (PIP3). Dephosphorylation of PIP3 is an important factor in cell growth, proliferation, apoptosis, and survival. Dephosphorylation of PIP3 blocks Akt signaling that can lead to higher activity of pro-apoptotic molecules such as Bad and Caspase-9. PTEN is considered a major tumor suppressor and its mutations are reported in several human tumors. Over seventy different types of PTEN mutation have been recorded in patients with Cowden syndrome. PTEN -/- mice exhibit higher levels of 3 -phosphorylated phospholipids and die during embryogenesis due to the failure of developmental apoptosis. Lysine 13 is one of the conserved sites for ubiquitination and monoubiquitination is essential for the movement of PTEN from cytoplasm to the nucleus. NEDD4-1 is identified as E3 ligase that induces both mono- and poly-ubiquitination of PTEN.

This rabbit polyclonal antibody detects phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN monoubiquitinated on Lysine 13. Immunogen sequence is conserved, hence, it is expected to react with all 3 isoforms.

KLH-conjugated linear peptide corresponding to 12 amino acids surrounding ubiquitinated Lysine 13 from the N-terminal region of human PTEN.

Epitope: N-terminus

Research Category
Signaling
Peptide Inhibition Analysis: A 1:1500 dilution from a representative lot blocked PTEN mono-ubiquitinated K13 in HEK293 cell lysate.



Anti-PTEN mono-ubiquitylated K13, Cat. No. ABS533, is a rabbit polyclonal antibody that detects phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PTEN mono-ubiquitinated K13 in 10 µg of HEK293 cell lysate.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

antibody small pack of 25 μL

inhibition assay: suitable (peptide)
western blot: suitable

IgG

NP_000305.3

P60484

ambient

human ... PTEN(5728), TEP1(7011)

~65 kDa observed; 47.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1