Anti-Ric-8B/Synembryn-B Antibody

32160702

NA.41

Synembryn-B (UniProt Q80XE1; also known as Protein Ric-8B, Resistance to inhibitors of cholinesterase 8B) is encoded by the Ric8b (also known as BC051080) gene (Gene ID 237422) in murine species. Ric-8A and Ric-8B stimulate nucleotide exchange of all Gα subunit classes by stabilizing the Gα nucleotide-free transition state. Ric-8A acts upon Gαi/q/13, while Ric-8B targets Gαs/olf. In addition to their function as guanine nucleotide exchange factors (GEFs), Ric proteins also act as molecular chaperones that directly participate in the posttranslational folding of nascent Gα subunits to promote G-protein biosynthesis and membrane association. Ric-8 knockdown reduces Gα steady-state abundances and overexpression of Ric-8 proteins enhances cellular Gα levels. In Ric-8A– or Ric-8B–delicient ES cells, newly produced Gα subunits are defective in the initial association with an internal membrane, resulting in a ∼10-fold faster turned over rate than in WT cells.

Expected to react with all murine and human spliced isoforms (UniProt Q80XE1 & Q9NVN3).

Recombinant protein corresponding to mouse Ric-8B/Synembryn-B.

This Anti-Ric-8B/Synembryn-B Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Ric-8B/Synembryn-B.
Western Blotting Analysis: A representative lot detected Ric-8B protein in murine embryonic stem (mES) cells (mESCs) derived from wild-type (WT) and Ric-8A−/− mice, but not mESCs from Ric-8B−/− mice (Gabay, M., et al. (2011). Sci Signal.4(200):ra79).
Western Blotting Analysis: A representative lot detected the endogenous Ric-8B protein in rabbit reticulocyte lysates (RRL) as well as recombinant murine Ric-8B protein (Chan, P., et al. (2013). Proc Natl Acad Sci U S A. 110(10):3794-3799).
Immunoprecipitation Analysis: A representative lot immunoprecipitated the endogenous Ric-8B protein from rabbit reticulocyte lysates/RRL (Chan, P., et al. (2013). Proc Natl Acad Sci U S A. 110(10):3794-3799).

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected Ric-8B/Synembryn-B in 10 µg of Jurkat cell lysate.

Unpurified

rabbit

100

serum

primary antibodies

polyclonal

rabbit, human, mouse

immunoprecipitation (IP): suitable
western blot: suitable

NP_898995

Q80XE1

dry ice

~63 kDa observed. Uncharacterized band(s) may appear in some lysates.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1