Anti-SHOC2 Antibody

32160702

NA.41

The Leucine-rich repeat protein SHOC-2 (SHOC2) is the regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and plays a key role in MAPK pathway activation. Upon RAS activation, SHOC2 acts as a positive modulator of the RAS-MAPK signaling via specifically allowing PP1c to dephosphorylate the ′Ser-259′ inhibitory site of RAF1 kinase and stimulate RAF1 activity. SHOC2 localizes to cytoplasm when inactive but rapidly translocates to the nucleus when activated, such as during growth factor stimulation. Thus, as expected, SHOC2 also participates in FGF receptor signaling. Molecularly, SHOC2 is a scaffold protein that brings together activated Ras (activated via G-protein coupled receptors and the interaction of GTP to GDP) and Raf, which then facilitates the transfer of the phospho-Raf phosphate to other proteins for the activation of the MAP Kinase pathway for gene activation. Defects in SHOC2 lead to a type of multi-symptomatic disorder termed the Noonan Syndrome Loss of Anagen Hair (NSLAH) that affects everything from skeletal formation to facial features and many organs including the brain, skin, muscles, and heart. EMD Millipore’s Anti-SHOC2 rabbit polyclonal antibody has been tested in western blotting and immunohistochemistry in human samples.

KLH-conjugated linear peptide corresponding to human SHOC2.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected SHOC2 in human spleen tissue.
Detect Leucine-rich repeat protein SHOC-2 using this rabbit polyclonal antibody, Anti-SHOC2 Antibody validated for use in western blotting & IHC.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SHOC2 in 15 µg of Jurkat cell lysate.

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Antigen Affinity Purified

Control
Jurkat cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

immunohistochemistry: suitable
western blot: suitable

Q9UQ13

wet ice

human ... SHOC2(8036)

~65 kDa observed. Uniprot describes 2 isoforms at ~60 kDa and ~65 kDa Uncharacterized bands may appear in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2