Anti-STEAP4 Antibody

32160702

NA.41

Metalloreductase STEAP4 (STEAP4), also known as Dudulin-4, Six-transmembrane epithelial antigen of prostate 4, Tumor necrosis factor-alpha-induced adipose related protein, and encoded by the gene name STEAP4 and TIARP, is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP4 inhibits anchorage-independent cell proliferation and uses NAD+ as acceptor. STEAP4 is associated with obesity and insulin-resistance, and might be involved in inflammatory arthritis, through the regulation of inflammatory cytokines. STEAP4 has been shown to play an important role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses. STEAP4 interacts with and phosphorylates PTK2/FAK1. STEAP4 is expressed in white and brown adipose tissues cells, as well as in muscle and liver cells. In addition, STEAP4 has been detected in joints and spleens of arthritic mice.

Recombinant protein corresponding to mouse STEAP4.

Immunohistochemistry Analysis: A representative lot detected STEAP4 in Adipose tissue, prostate tissue, and prostate cancer tissue (Freyhaus, H., et al. (2012) Cell Metabolism. 16:81-89).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Anti-STEAP4 is an antibody against STEAP4 for use in WB, IH.

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected STEAP4 in 10 µg of mouse liver tissue lysate.

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Concentration: Please refer to lot specific datasheet.

rabbit

100

serum

primary antibodies

polyclonal

mouse, human

immunohistochemistry: suitable
western blot: suitable

NP_473439

Q923B6

dry ice

~58 kDa observed. The calculated molecular weight is 52 kDa, however STEAP4 has been shown as a ~58 kDa band in some western blots (Freyhaus, H., et al. (2012) Cell Metabolism. 16:81-89).

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1