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Anti-SYNE1 Antibody, clone 7A12 (MANNES1A)

产品编号:4114943
规格:clone 7A12, from mouse
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4114943100 μG5350
产品别名

Anti-SYNE1 Antibody, clone 7A12 (MANNES1A)

Nesprin-1, Enaptin, Myne-1, Myocyte nuclear envelope protein 1, Nuclear envelope spectrin repeat protein 1, Synaptic nuclear envelope protein 1, Syne-1

基本信息
eCl@ss
32160702
General description【一般描述】
Nesprin-1 (UniProt Q8NF91; also known as Enaptin, Myne-1, Myocyte nuclear envelope protein 1, Nuclear envelope spectrin repeat protein 1, Synaptic nuclear envelope protein 1, Syne-1) is encoded by the SYNE1 (also known as C6orf98, EDMD4, KIAA0796, KIAA1262, KIAA1756, MYNE1, SCAR8) gene (Gene ID 23345) in human. SYNE1 gene encodes multiple intracellular linker/scaffold molecules as a result of alternate start codons usage and splicings. Structurally, nesprins have a central rod domain composed of spectrin repeats. Nesprin-1 isoforms giant/4/5/6/7/10 and nesprin-2 isoforms giant/p32CH/NUANCE-N-33 have two N-terminal CH (calponin homology) domains that bind the actin cytoskeleton. Nesprin-1 isoforms giant/beta/alpha/4/beta 2/alpha 2/myne-1 and Nesprin-2 isoforms giant/2/epsilon2/beta1/alpha1/alpha2/gamma/beta2/FLJ56122/FLJ55476/epsilon1 contain a C-terminal transmembrane KASH (Klarsicht-ANC-Syne-homology) domain that resides in the outer nuclear membrane and bind across the luminal space to the SUN (Sad1, UNC84) type II inner nuclear membrane proteins, SUN1 and SUN2, to form LINC (Linker of Nucleoskeleton and Cytoskeleton) complexes that link the cytoskeleton and the nucleus. Human SYNE1 gene is among the top ten loci conferring risk for the five major psychiatric disorders, schizophrenia, bipolar disorder (BD), major depression, attention deficit-hyperactivity disorder (ADHD), and autism spectrum disorder (ASD), as well as multiple other neurological and neuromuscular disorders.
Specificity【特异性】
Epitope region is present in, human isoforms 1 (Giant; Enaptin)/2 (Beta)/3 (Alpha)/4/5/8 (Beta 2)/9 (Alpha 2)/11 (myne-1; 131kDa), mouse isoforms 1 (Giant; Enaptin)/2/3 (Syne-1A)/4 (Syne-1B)), and rat Nesprin-1 (UniProt Q8VHJ9), but absent from human spliced isoforms 6/7/10 (drop1)/12 (GSRP-56; 56 kDa), mouse spliced isoform 5 (Enaptin-165), or rat spliced isoform CPG2 (UniProt Q63128) reported by UniProt (Q8NF91, Q6ZWR6, Q8VHJ9, Q63128). Clone 7A12 does not reacts with nesprin-2, but stained centrosome in acetone/methanol-fixed HeLa cells due to uncharacterized cross-reactivity (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Immunogen【免疫原】
GST-tagged recombinant human SYNE1 isoform Beta C-terminal 121-a.a. fragment fused to full-length isoform Alpha sequence (UniProt Q8NF91-2 andQ8NF91-3).
Application【应用】
Research Category
Cell Structure
Western Blotting Analysis: 10 µg/mL from a representative lot detected SYNE1 spliced isoform Giant (Enaptin) in lysate from a 4-day old human skeletal myotube culture (Courtesy of Glenn E. Morris, Ph.D., Wolfson Centre for Inherited Neuromuscular Disease, Oswestry, United Kingdom).

Immunocytochemistry Analysis: A representative lot immunostained the nucleus of acetone/methanol-fixed rat aortic vascular smooth muscle cells (VSMCs) by fluorescent immunocytochemistry (Duong, N.T., et al. (2014). PLoS One. 9(4):e94380).

Immunocytochemistry Analysis: A representative lot detected SYNE1 immunoreactivity predominantly in the nucleus by fluorescent immunocytochemistry staining of acetone/methanol-fixed human skin fibroblasts regardless of their emerin expression. SYNE1 immunoreactivity was redistributed to ER in the absence of lamin A/C in Lamn-/- mouse embryonic fibroblasts (MEFs) (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).

Immunocytochemistry Analysis: A representative lot immunostained multinucleate myotube nuclear envelopes, while only very weak or little SYNE1 immunoreactivity was seen among mononucleated myoblasts in myoblast cultures undergoing differentiation (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).

Western Blotting Analysis: A representative lot detected multiple SYNE1 spliced isoforms (including Giant/Enaptin, Beta, and Alpha) in human muscle tissue, skeletal muscle cells and dermal fibroblasts, as well as in Lmna-/- mouse embryonic fibroblasts (MEFs) (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).

This mouse monoclonal Anti-SYNE1, clone 7A12 (MANNES1A) , Cat. No. MABT843 is validated for use in and Immunocytochemistry, Western Blotting and Western Blotting, for the detection of Nesprin-1.
Quality【质量】
Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Physical form【外形】
Protein G purified.
Format: Purified
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
7A12, monoclonal
species reactivity
rat, rabbit, mouse, human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NP_892006
UniProt accession no.【UniProt登记号】
Q8NF91
shipped in【运输】
ambient
Gene Information
human ... SYNE1(23345)
产品说明
Target description【目标描述】
~1,000 kDa (Giant; Enaptin) observed. 1,011/380.4/112.4/1,005/167.3/367.9/111.6/131.2 kDa (human isoforms 1 (Giant; Enaptin)/2 (Beta)/3 (Alpha)/4/5/8 (Beta 2)/9 (Alpha 2)/11 (myne-1; 131kDa)), 1010/111,3/108.8/1010 kDa (mouse isoforms 1 (Giant; Enaptin)/2/3 (Syne-1A)/4 (Syne-1B)), and 325.1 kDa (rat Nesprin-1; UniProt Q8VHJ9) calculated. Uncharacterized bands may be observed in some lysate(s).
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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