Anti-Tapasin Antibody, clone 7F6

32160702

NA.41

Tapasin (TPN or TPSN) is also known as NGS-17, TAP-associated protein and TAP-binding protein. Tapasin plays a role in MHC class I peptide loading and assembly, and is involved in associating MHC class I with transporter associated with antigen processing (TAP). Tapasin is found in neutrophils, predominantly within fully differentiated cells.

This antibody recognizes the Lumenal domain of Tapasin.

Linear peptide corresponding to human Tapasin.

Detect Tapasin using this rat monoclonal antibody, Anti-Tapasin Antibody, clone 7F6 validated for use in western blotting & Flow Cytometry.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Tapasin in 10 µg of A431, HEK293, HepG2, HUVEC, Jurkat, and PC12 cell lysates.

Flow Cytometry Analysis: 4 µg from a representative lot detected Tapasin in 1X10E6 PBMC cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA transfected MJS cell lysate (Hislop, A. D., et al. (2007). J Exp Med. 204(8):1863-1873.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in HeLa cell lysate (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA4M transfected MJS cell lines (Horst, D., et al. (2009). J Immunol. 182(4):2313-2314.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in WB (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tapasin in 10 µg of HeLa cell lysate.

Protein G Purified
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

rat

purified antibody

primary antibodies

7F6, monoclonal

human

flow cytometry: suitable

IgG1κ

np_003181

O15533

wet ice

human ... TAPBP(6892)

~48 kDa observed

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WGK 1