Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85

32160702

NA.41

The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.

This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.

SPECIES REACTIVITIES:

Refer to comments regarding specificity

Epitope: blood group antigen Ok(a)
2102Ep human Embryonal Carcinoma Cells

Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.
Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research
FACS Analysis: a starting range of 5-20 μg/ml is suggested.

Immunoprecipitation

Immunoblotting

Optimal working dilutions must be determined by the end user.

Format: Purified
Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide

Control
Hemopoietic cells and a wide range of normal human tissues

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

TRA-1-85, monoclonal

human

Chemicon^®

flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

IgG1

wet ice

35-69 kDa

Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2