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Anti-Ubiquitin-conjugating enzyme E2 A Antibody
| 产品编号： | 4115777 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Ubiquitin-conjugating enzyme E2 A Antibody
Ubiquitin-conjugating enzyme E2 A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Ubiquitin-conjugating enzyme E2 A (EC 6.3.2.19; UniProt P49459; also known as hHR6A, HR6A, RAD6 homolog A, Ubiquitin carrier protein A) is encoded by the UBE2A (also known as MRXS30, MRXSN, RAD6A, UBC2) gene (Gene ID 7319) in human. UBE2A accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2A is involved in transcription regulation by catalyzing the monoubiquitination of histone H2B Lys120 to form H2BK120ub1. H2BK120ub1 serves as a specific signal for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, as well as a prerequisite for H3K4me and H3K79me formation. In vitro, UBE2A catalyzes both Lys11 and Lys48-linked polyubiquitination. UBE2A is also involved in UV-damaged DNA repair and aberrant UBE2A can cause mental retardation syndromic X-linked Nascimento-type (MRXSN). |
| Specificity【特异性】 | Expected to react with all three spliced isoforms. |
| Immunogen【免疫原】 | Epitope: Near C-terminus KLH-conjugated linear peptide corresponding to human Ubiquitin-conjugating enzyme E2 A sequence near the C-terminus. |
| Application【应用】 | This Anti-Ubiquitin-conjugating enzyme E2 A Antibody is validated for use in Western Blotting for the detection of Ubiquitin-conjugating enzyme E2 A. Research Sub Category Nuclear Receptors Research Category Epigenetics & Nuclear Function |
| Quality【质量】 | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ubiquitin-conjugating enzyme E2 A in 10 µg of HeLa cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human, mouse, rat |
| species reactivity (predicted by homology) | chicken (based on 100% sequence homology), horse (based on 100% sequence homology), sheep (based on 100% sequence homology), primate (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_003327 |
| UniProt accession no.【UniProt登记号】 | P49459 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... UBE2A(7319) |
产品说明
| Target description【目标描述】 | ~17 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
