Anti-Versican Antibody, clone 8D8.1

32160702

NA.41

Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.

This antibody recognizes the C-terminus of Veriscan.

KLH-conjugated linear peptide corresponding to the C-terminus of human Veriscan.
Epitope: C-terminus

Research Category
Cell Structure
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human cerebellum, and mouse brain and embryonic skeletal muscle.

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Versican in mouse leg muscle tissue lysate.
Detect Versican using this mouse monoclonal antibody, Anti-Versican Antibody, clone 8D8.1 validated for use in IHC & western blotting.
Research Sub Category
ECM Proteins

Evaluated by Immunohistochemistry in human thalamus tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human thalamus tissue lysate.

Purified by Ion-Exchange Chromatography
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Control
Human thalamus tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

primary antibodies

8D8.1, monoclonal

mouse, human

immunohistochemistry: suitable
western blot: suitable

IgMκ

NP_00119808

P13611

wet ice

372 kDa calculated. Uniprot describes three isoforms at 265 kDa, 182 kDa, and 74 kDa Versican is known to be secreted following valvular interstitual cell (VIC) injury and repair (Carthy, J., et al. (2012). Cardiovac. Pathol. 21(2):74-82.).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2