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Anti-Vesicular Glutamate Transporter 1 Antibody
产品别名
Anti-Vesicular Glutamate Transporter 1 Antibody
Solute carrier family 17 member 7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, vGluT1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate. Vesicular glutamate transporters pack the neurotransmitter into synaptic vesicles so that they can be released into the synapse. VGLUTs are dependent on a proton gradient that they create by hydrolysing adenosine triphosphate (ATP). VGLUTs have only between one hundredth and one thousandth the affinity for glutamate that EAATs have. |
| Specificity【特异性】 | Reacts with VGLUT1 (Vesicular Glutamate Transporter 1). |
| Immunogen【免疫原】 | Recombinant protein from rat VGLUT1. |
| Application【应用】 | Research Sub Category Ion Channels & Transporters Neuronal & Glial Markers Research Category Neuroscience This Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Cat. No. MAB5502, is validated for use in IH, WB for the detection of VGluT1. Immunohistochemistry: A previous lot of this antibody was tested on IH. Optimal working dilutions must be determined by end user. |
| Quality【质量】 | Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot Analysis: 1:500 dilution of this lot detected Vesicular Glutamate Transporter 1 on 10 μg of Mouse Brain lysates. |
| Physical form【外形】 | Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.1% sodium azide. Format: Purified Protein A purified |
| Analysis Note【分析说明】 | Control Brain VGLUT1 Control Peptide AG208 |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 3C10.2, monoclonal |
| species reactivity | mouse |
| species reactivity (predicted by homology) | rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG1 |
| NCBI accession no.【NCBI登记号】 | NP_446311 |
| UniProt accession no.【UniProt登记号】 | Q62634 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 60 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
