Cell-in-a-Box^® Decapsulation Solution

liquid

ambient

room temp

Cell-in-a-Box^® cell encapsulation technology allows one to encapsulate cells using proprietary nanomaterials that can be performed manually using a simple disposable system. The kit allows researchers to quickly and easily encapsulate their own cells for further laboratory studies such as testing for cell survival, viability, growth, release of biomolecules in cell therapy and 3D cell culture applications. The proprietary Cell-in-a-Box^® porous cellulose beads are approximately 2 mm in diameter and have a pore size which allows nutrients to enter and waste products and biomolecules as large as antibodies to leave the capsule unhindered, yet cells are unable to pass in or out.

The Cell-in-a-Box^® Decapsulation Solution is a cell-friendly reagent that gently dissolves the membrane of porous bead capsules generated with the CIB001 kit, allowing the user to recover and harvest the encapsulated cells for further downstream analysis and processing, such as cell counting, DNA extraction, and fluorescence imaging.

1. Place 10 capsules made using the CIB001 Cell encapsulation kit into a 6-well plate, in 7.2 ml of cell culture medium or PBS.
2. Take 800 ul of Decapsulation Solution (10X) and add it into the well, in order to make a 1X working solution.
3. Place the plate on a horizontal shaker under gentle shaking for 15-30 minutes. Observe regularly with the naked eye or under the microscope to determine when the decapsulation process is sufficiently completed
4. Pipette all the contents into a 15 ml centrifuge tube and centrifuge at 200-300g for 5 minutes.
5. Discard the supernatant and resuspend the cell pellet with 8ml fresh medium or PBS.
6. Repeat step 4.
7. Remove the supernatant and resuspend in an appropriate volume of cell culture medium or PBS for specific downstream application.

Notes:
The Decapsulation Solution has been optimized for the decapsulation of capsules as described in the procedure above. Should there be a need to deviate from it, the procedure will need to be re-optimized at the users own discretion. As a general rule, the ratio of 10 capsules to 8 ml of 1X Decapsulation Solution should be maintained. Increasing the number of capsules used might lead to incomplete decapsulation, while increasing the concentration of Decapsulation Solution might cause some loss of cell viability.

Cell-in-a-Box is a registered trademark of Austrianova

12 - Non Combustible Liquids

WGK 2