ColorWheel^® Antibody-Ready mFluor Violet 450 Dye

200

ColorWheel^®

lyophilized

flow cytometry: suitable

λ_ex 406 nm; λ_em 445 nm

ambient

2-8℃

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

mFluor Violet 450 is suitable for multi-color flow cytometry applications. It has an excitation max at 406 nm and emission max at 445 nm and displays an excitation coefficient at 35,000 cm^-1 M^-1. These spectral properties make them an excellent replacement for Pacific Blue labeling dye. It has reasonable stability, good reactivity and selectivity with amino groups in proteins. It is suitable for labeling monoclonal and polyclonal antibodies.

Quality Control Testing:

Flow Cytometry: Excitation: 406 nm, Emission: 445 nm

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

CWDS-MF450-25UL Set Components

CWD-MF450-25UL - ColorWheel^® Antibody-Ready mFluor Violet 450 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - ColorWheel^® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.