COMP/TSP5 human

NA.32

Cartilage oligomeric matrix protein (COMP) also known as Thrombospondin-5 (TSP5), EDM1, EPD1, MED, PSACH, THBS5, which belongs to the thrombospondin family. COMP / TSP5 contain 4 EGF-like domains, 1 TSP C-terminal (TSPC) domain, 8 TSP type-3 repeats. Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. COMP may play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. COMP can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Thrombospondin-5 could play a role in the pathogenesis of osteoarthritis. COMP is a marker of cartilage turnover.
Cartilage oligomeric matrix protein (COMP), also known as thrombospondin 5 (TSP5), is a key constituent of the extracellular matrix of the musculoskeletal system. It is encoded by the gene mapped to human chromosome 19p13.11. COMP/TSP5 is a fifth member of TSP family. It is a pentameric protein, characterized with an N-terminal coiled- coil oligomerization domain, four type II (EGF-like) domains, eight type III calmodulin like repeats (CLR) and a C-terminal globular domain (CTD).

Cartilage oligomeric matrix protein (COMP) plays a vital role in chondrocyte attachment, differentiation and cartilage extracellular matrix assembly. COMP levels vary in cartilage of osteoarthritis and rheumatoid arthritis patients compared with healthy individuals. Thus, COMP levels in serum and synovial fluid can be used as a biomarker for these diseases. Polymorphism in the gene leads to the development of two autosomal dominant skeletal dysplasias named, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia type 1 (MED1).

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.

human

expressed in HEK 293 cells

6-His tagged (C-terminus)

≥95% (SDS-PAGE)

lyophilized

1 μg

calculated mol wt 82 kDa
observed mol wt 110-130 kDa (DTT-reduced. Protein migrates due to glycosylation. Gln 21 is the predicted N-terminal.)

pkg of 10 and 50 μg

<1 EU/μg endotoxin (LAL test)

P49747

wet ice

−20℃

human ... COMP(1311)

11 - Combustible Solids

WGK 3