Duolink^® flowPLA Detection Kit-Violet

Duolink^®

flow cytometry: suitable
immunofluorescence: suitable
proximity ligation assay: suitable

λ_ex 390 nm; λ_em 476 nm

suitable for fluorescence

dry ice

−20℃

Based on proximity ligation assay (PLA), the Duolink^® PLA technology allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells.

Duolink^® flowPLA Detection Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink^® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest, a pair of PLA probes (one 100RXN PLUS and one 100 RXN MINUS), wash buffer and a Duolink^® flowPLA Detection Kit. The flowPLA Kits are available with 5 different fluorophores: Violet, Green, Orange, Red, or FarRed. The flowPLA Kits contain all the necessary reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, primary antibodies, and corresponding PLA Probes.

Follow the Duolink^® PLA Flow Cytometry Protocol to use this product.

Visit our Duolink^® PLA Flow Cytometry page on how to run a Duolink^® flow experiment, applications, troubleshooting, and more.

Specificity

Violet Fluorescence Detection Reagents
Use appropriate laser for λex 390 nm excitation
Use appropriate filter for λem 476 nm emission

Application Note

Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended.

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This product is comprised of the following:

• 5x Detection Solution - Violet (DUO84005)
• 5x Ligation Buffer (DUO82009)
• 5x Amplification Buffer (DUO82050)
• Ligase (1U/μL)
• Polymerase (10U/μL)

See datasheet for more information.

Duolink is a registered trademark of sigma-aldrich Co. LLC

10 - Combustible liquids

WGK 2