Duolink^® PLA^® Probe Rabbit IgG Isotype Control PLA PLUS from rabbit

rabbit serum (polyclonal)

200

affinity purified immunoglobulin (Isotype control antibody)

Duolink^®

proximity ligation assay: suitable

suitable for brightfield
suitable for fluorescence

wet ice

2-8℃

An immunoglobulin G (IgG) isotype control antibody with the Duolink^® PLA PLUS oligonucleotide specific for rabbit IgG. This is a convenience product to be used in place of a rabbit primary antibody to determine non-specific background signal and optimal antibody dilution. The anti-rabbit PLA probe PLUS is not necessary when using this product, but must be used in combination with a MINUS PLA probe.

Duolink^® proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink^® In Situ Fluorescence Protocol and the Duolink^® In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting, and more.

Application Note
To perform a complete Duolink^® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Isotype control PLA probes are used in lieu of a primary antibody in a Duolink^® PLA in situ experiment to determine background signal contributed by non-specific binding of the primary antibody. Isotype control PLA probes can also be used to determine the optimal titer conditions of primary antibody PLA probes.

A MINUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

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PLA is a registered trademark of sigma-aldrich Co. LLC

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