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E. coli Fluorescent Particles
产品编号: | 4118382 |
规格: | Green E. coli conjugate for phagocytosis |
包装规格: | 500 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品性质
Quality Level【质量水平】 | 100 |
concentration【浓度】 | ~20 mg/mL |
storage temp.【储存温度】 | 2-8℃ |
suitability【适用性】 | Suitable for inducing phagocytosis and screening activators and/or inhibitors of phagocytosis and Toll-like receptor ligands (TLR) in mammalian cells. |
基本信息
General description【一般描述】 | Phagocytosis is a cellular process for ingesting and eliminating microorganisms, foreign substances, and apoptotic cells. It is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as pathogens in phagocytosis assays. |
Application【应用】 | E. coli fluorescent particles were prepared by conjugating BL21DE3 E. coli particles with a bright green fluorescent molecular probe. The particles are ready-to-use phagocytosis-inducing particles for fluorescent imaging, microplate reader (Ex/Em = 490/520 nm) and flow cytometric analysis of phagocytosis. We recommend using 5 μL of E. coli fluorescent particles per 5 x 105 of viable cells. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |