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EED human
产品编号: | 4118416 |
规格: | recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE) |
包装规格: | 50 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
EED human
Rinchik 5
embryonic ectoderm development
WAIT1
HEED
WD protein associating with integrin cytoplasmic tails 1
基本信息
NACRES | NA.32 |
General description【一般描述】 | EED (ectoderm development protein) is the part of the polycomb repressive complex 2 (PRC2) where it interacts with EZH2 (enhancer of zeste homolog 2). The protein has seven WD domains (β-transducin domains). The EED gene is mapped to human chromosome 11q14. Human Embryonic Ectoderm Development (hEED), also known as WAIT-1, GenBank Accession No. NM_003797, amino acids 2-end with N-terminal FLAG-tag, MW = 51kDa, expressed in Sf9 cells via a baculovirus expression system. |
Biochem/physiol Actions【生化/生理作用】 | EED (ectoderm development protein) is required for proper EZH2 (enhancer of zeste homolog 2)-mediated methyltransferase activity. It also associates with PRC1 (polycomb repressive complex 1) and helps in bringing PRC1 to H3K27me3 (trimethylated lysine residue at position 27 in the protein histone H3) loci. EED also increases PRC1-mediated H2A (histone 2A) ubiquitin E3 ligase activity. Mutations in the EED gene are associated with overgrowth, intellectual disability and dysmorphic features. Change in the activity of EED is linked with the pathogenesis of myeloid disorders. |
Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol. |
Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in baculovirus infected insect cells |
Assay【测定】 | ≥90% (SDS-PAGE) |
form【形式】 | aqueous solution |
mol wt【分子量】 | 51 kDa |
packaging【包装】 | pkg of 50 μg |
concentration【浓度】 | >0.02 mg/mL |
NCBI accession no.【NCBI登记号】 | NM_003797 |
UniProt accession no.【UniProt登记号】 | O75530 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... EED(8726) |