EGFR (T790M C797S L858R), Active

expressed in baculovirus infected Sf9 cells

70-95% (SDS-PAGE)

frozen liquid

34-46 nmol/min·mg (radiometric)

~89-100 kDa

0.1 μg/μL

NM_005228

dry ice

−70℃

human ... EGFR(1956)

Useful for kinase assay.

Recombinant human EGFR (T790M C797S L858R) (668-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival ⁽¹⁾. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms ⁽²⁾. Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

10 - Combustible liquids

WGK 1