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Histone H3 (2-58) human
| 产品编号： | 4119188 |
| 规格： | recombinant, expressed in E. coli, ≥70% (SDS-PAGE) |
| 包装规格： | 500 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Histone H3 (2-58) human
Histone H3.1
HIST1H3E
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | The mammalian chromatin is present in nucleosomes, made of histone octamers (H2A,H2B,H3,H4)2. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A). Human Histone 3 (GenBank Accession No. NM_003532), (amino acid 2-58) with N-terminal GST tag, MW = 32kDa, expressed in an Escherichia coli expression system. |
| Application【应用】 | Suitable substrate for histone methyltransferases |
| Biochem/physiol Actions【生化/生理作用】 | Histone proteins are basic building blocks of chromatin. H3.1 (also referred to as HIST1H3E) is mainly expressed in the S phase and is termed as replication-dependent histone. Selective methylation of H3.1 controls replication in heterochromatin area. |
| Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥70% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 32 kDa |
| packaging【包装】 | pkg of 500 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | >0.02 mg/mL |
| NCBI accession no.【NCBI登记号】 | NM_003532 |
| UniProt accession no.【UniProt登记号】 | P68431 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... HIST1H3E(8353) |
