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Human CRISPR Activation Calabrese Library
产品性质
| packaging【包装】 | pkg of 10 vials (5x200µL aliquots of each component) |
| concentration【浓度】 | ≥5x108 VP/ml (via p24 Assay) |
| application(s) | CRISPR |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
基本信息
| General description【一般描述】 | The Human CRISPR activation library (Calabrese) activates over 18,000 human genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018). Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative. |
| Application【应用】 |
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| Features and Benefits【特点和优势】 |
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| Components【组分】 | 2 Subpools with a minimum concentration of 5x108 VP/mL(via p24 assay) PCRISPR001A - Human CRISPR Activation Calabrese Library Set A PCRISPR001B - Human CRISPR Activation Calabrese Library Set B |
| Principle【原理】 | The most effective CRISPRa system uses the CRISPR Synergistic Activation Mediator (SAM) complex. This platform combines VP64-dCas9 with a modified guide. In addition, the gRNA contains two aptamers that recruit additional co-activators, p65 and HSF1, enhancing target gene activation. CRISPRa can also serve as a complementary approach to loss-of-function (LOF) genetic screens by enriching for a different set of genes responsible for a phenotype CRISPRa screens show little-to-no off-target activity and can be used to study genes that are difficult to characterize using only LOF approaches because of their high copy number. |
| Legal Information【法律信息】 | LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
