Human Ferritin ELISA Kit

NA.32

The Human Ferritin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ferritin in serum, plasma, cell culture supernatants and urine.
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).

Recombinant Human Ferritin

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human ferritin ELISA kit. has been used to measure the ferritin secreted in supernatants from THP-1 (tamm-horsfall protein 1) cells that were sequentially treated with lipopolysaccharides (LPS) and LLOMe (a lysosome disruption agent).
Human Ferritin ELISA Kit has been used to estimate the amount of ferritin in cellular suspension.

Ferritin/ ferritin heavy chain 1 (FTH1) is a cytoplasmic protein. It acts as a cargo for transport to autophagosomes for degradation. FTH1 maintains the cellular redox status by storing the intracellular iron in a non-toxic and readily available form. It acts as a key antioxidant molecule in various physiologic and pathologic conditions, such as apoptosis, inflammation, vascular protection and neurodegeneration. FTH1 alone or along with ferritin light chain is implicated to detect cancer cells, stem cells, fibroblasts and dendritic cells.
Ferritin mediates iron metabolism and plays a key role in redox homeostasis. Ferritin possesses ferroxidase activity and maintains iron in its non-toxic form. Ferritin acts as an iron-storage protein and stores excessive intracellular iron that is not utilized for the production of heme. Also, iron is released from ferritin upon degradation.

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

组份可单独销售
RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
RABSTOP3 ELISA Stop Solution (Item I)
RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
RABWASH4 20X Wash Buffer (Item B)

human

kit of 96 wells (12 strips x 8 wells)

ELISA: suitable
capture ELISA: suitable

sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.81-200 ng/mL

colorimetric

wet ice

−20℃

human ... FTH1(2495)

GHS05

Warning

H290

P234 - P390

Met. Corr. 1

8A - Combustible, corrosive hazardous materials

WGK 3