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Human OKSG-cMyc Simplicon® RNA
产品编号: | 4119354 |
规格: | EMD Millipore’s SimpliconTM RNA Reprogramming Kit is a safe and efficient method to generate integration free, virus-free human iPS cell using a single transfection step of self-replicating synthetic RNA for Oct4, Klf4, Sox2, Glis1 and c-M |
包装规格: | 1 KIT |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
eCl@ss | 32161000 |
NACRES | NA.51 |
General description【一般描述】 | Various methods utilizing viruses, DNA, RNA, miRNA and protein have been developed to generate integration-free induced pluripotent stem cells (iPSCs). Disadvantages to existing methods include: (1) low reprogramming efficiency (i.e. DNA and protein), (2) a lengthy requirement for negative selection and subcloing steps to remove persistent traces of the virus (i.e. Sendai virus)1 and (3) daily transfections of cells using four synthetic mRNAs over a 14 day period (i.e. mRNA based). EMD Millipore′s Simplicon® OKSG-cMyc RNA uses a safe and efficient method to generate integration- and virus-free human iPSCs using a single transfection step. The technology utilizes a single self-replicating Venezuelian equine encephalitis (VEE) RNA species that expresses reprogramming factors (RF) ORFs3. The Simplicon RNA replicon is a synthetic polycistronic VEE-RF RNA expressing five reprogramming factors (OKSG-cMyc; Oct4, Klf4, Sox2, Glis1 and cMyc), capable of self-replicating in a limited number of cell divisions. The 5-factor OKSG-cMyc Simplicon is especially useful for iPSCs generation from somatic cells that are more difficult to reprogram (i.e. slower proliferating cells or aged somatic cells). |
Application【应用】 | Research Category Stem Cell Research Advantages of the Simplicon® OKSG-cMyc RNA:
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Components【组分】 | 1. VEE-OKSG-cMyc RNA: (Part No. CS221303) One (1) vial containing 10 L of RNA (1 ug/uL). Store at -80℃. 2. B18R-E3L RNA: (Part No. CS224503) One (1) vial containing 10 L of RNA (1 ug/uL). Store at -80℃. |
Quality【质量】 | Tested to confirm the generation of iPS cells from p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed. |
Legal Information【法律信息】 | SIMPLICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
technique(s) | cell culture | stem cell: suitable |
input | sample type induced pluripotent stem cell(s) |
shipped in【运输】 | ambient |
产品说明
Storage and Stability【储存及稳定性】 | VEE-OKSG-cMyc and B18R RNAs: Stable for 4 months from date of receipt when stored appropriately at -80℃. For best recovery, quick-spin the vial after thawing on ice and prior to opening. Aliquot into sterile, nuclease-free eppendorf tubes on ice and store at -80℃. Limit repeated freeze-thaw cycles. Use in a sterile RNase-free environment. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |