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JMJD2b active human
| 产品编号： | 4119617 |
| 规格： | recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE) |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
JMJD2b active human
Jumonji domain-containing protein 2B
JHDM3B
JmjC domain-containing histone demethylation protein 3B
KIAA0876
Lysine-specific demethylase 4B (KDM4B)
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system. |
| Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Unit Definition【单位定义】 | One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37℃. |
| Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in baculovirus infected insect cells |
| Assay【测定】 | ≥40% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 57 kDa |
| packaging【包装】 | pkg of 100 μg |
| concentration【浓度】 | >0.02 mg/mL |
| NCBI accession no.【NCBI登记号】 | NM_015015 |
| UniProt accession no.【UniProt登记号】 | O94953 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... KDM4B(23030) |
