LPS, 大肠杆菌 O111:B4

32160405

NA.41

Lipopolysaccharide (LPS), also known as endotoxin, is present mainly in the cell wall of Gram-negative bacteria. It comprises three structural regions the O-side chain, a middle polysaccharide component, and Lipid A which contributes to the pyrogenic nature of LPS.

LPS, E. Coli O111:B4 has been used for priming macrophages to upregulate genes necessary for NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome activation. It has also been used to stimulate Raw264.7 cells before pull-down assay.

Lipopolysaccharide (LPS), an effective immunostimulant induces inflammatory and immune responses by activating Toll-Like Receptor 4 (TLR4). It also contributes to osteoclastogenesis in mice. LPS serves as a permeability barrier around the cell surface to restrict the entry of toxic molecules such as antibiotics and bile salts. It acts as a pyrogenic agent and eventually induces septic shock in host cells.

分析：<通过 Lowry 分析，蛋白质为3％。

PBS 中的液体，pH 7.4。

内毒素活性：通过凝胶法，每毫克 LPS 不少于 500,000 内毒素单位；生色(Cambrex 分析)毒性分析测量结果表明，LPS 约为 10 EU/纳克。

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