您的位置：首页 > 产品中心 > NEK3, active, GST tagged human
NEK3, active, GST tagged human
| 产品编号： | 4139086 |
| 规格： | PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution |
| 包装规格： | 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
NEK3, active, GST tagged human
HSPK36
MGC29949
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 mRNA is detected in all the proliferating cell lines with the amount not changing during the cell cycle. During Prolactin receptor signaling, VAV2 is phosphorylated and activated by NEK3. Overexpression of NEK3 in Chinese hamster ovary cells increases cytoskeletal re-organization in response to Prolactin while downregulation of NEK3 expression by siRNA block these effects. Prolactin also stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. |
| Physical form【外形】 | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note【制备说明】 | after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles |
| Legal Information【法律信息】 | PRECISIO is a registered trademark of sigma-aldrich Co. LLC |
产品性质
| recombinant【重组】 | expressed in baculovirus infected Sf9 cells |
| product line【产品线】 | PRECISIO® Kinase |
| Assay【测定】 | ≥70% (SDS-PAGE) |
| form【形式】 | buffered aqueous glycerol solution |
| Specific Activity | 112-152 nmol/min·mg |
| mol wt【分子量】 | ~86 kDa |
| NCBI accession no.【NCBI登记号】 | NM_002498 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... NEK3(4752) |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
