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产品性质
recombinant【重组】 | expressed in baculovirus infected Sf9 cells |
Assay【测定】 | 70-95% (SDS-PAGE) |
form【形式】 | frozen liquid |
mol wt【分子量】 | ~95-100 kDa |
concentration【浓度】 | 0.5 μg/μL (0.1 ug/uL each PAD) |
accession no.【登记号】 | BC025718 BC049947 NM_013358 NM_016233 NM_207421 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... PADI1(29943), PADI2(11240), PADI3(51702), PADI4(23569), PADI6(353238) |
基本信息
Application【应用】 | Useful for citrullination and Western blot. |
Biochem/physiol Actions【生化/生理作用】 | The five full length recombinant PAD proteins (1, 2, 3, 4 and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags. This mixture is ideally suited for in vitro citrullination of protein targets. |
Reconstitution【重悬】 | Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol. |
产品说明
Storage and Stability【储存及稳定性】 | Store in aliquots at -70℃. Avoid freeze/thaw cycles. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |