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PEST Active human
| 产品编号： | 4139653 |
| 规格： | recombinant, expressed in E. coli, ≥90% (SDS-PAGE) |
| 包装规格： | 20 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
PEST Active human
Lver regeneration-related protein LRRG084
PEST proteolytic signal containing nuclear protein
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Protein tyrosine phosphatase, Catalytic domain, (amino acids 2-300) (Gene bank accession number NM_002835) with N-terminal GST tag, MW = 61.4 kDa, expressed in an E. coli expression system. |
| Application【应用】 | Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. |
| Unit Definition【单位定义】 | One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃. |
| Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥90% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 61.4 kDa |
| packaging【包装】 | pkg of 20 μg |
| concentration【浓度】 | >0.02 mg/mL |
| NCBI accession no.【NCBI登记号】 | NM_002835 |
| UniProt accession no.【UniProt登记号】 | Q8WW12 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... PCNP(57092) |
