PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb

rabbit

100

diluted serum

primary antibodies

polyclonal

liquid

preservative

human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG

wet ice

−20℃

50 μL in Plastic ampoule

This PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).
Recognizes the ~95 kDa nibrin protein phosphorylated at Ser³⁴³ in δ-irradiated lymphoblastoid cells.
Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser³⁴³.

a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
Human

ELISA (1:16,000)

Immunoblotting (1:1000)

Immunoprecipitation (1 µl/500 µg cell extract)

Toxicity: Standard Handling (A)

Serum diluted in PBS.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
γ-irradiated lymphoblastoid cells

Gatei, M., et al. 2000. Nat. Genet.25, 115.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.
This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

WGK 1